Lam Sze Kwan, Han Qi Feng, Ng Tzi Bun
Department of Biochemistry, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China.
Biosci Rep. 2009 Jun 15;29(5):293-9. doi: 10.1042/BSR20080110.
A dimeric 62-kDa lectin exhibiting a novel N-terminal amino acid sequence was purified from caper (Capparis spinosa) seeds. The purification protocol involved anion-exchange chromatography, cation-exchange chromatography and, finally, gel filtration by FPLC on Superdex 75. Approx. 100-fold purification was achieved. The haemagglutinating activity of the lectin, which was stable in the pH range 1-12 and up to 40 degrees C, could be inhibited by D(+) galactose, alpha-lactose, raffinose and rhamnose at 1 mM concentration, by 25 mM L(+)-arabinose and by 100 mM D(+)GlcN (glucosamine). The lectin potently inhibited HIV-1 reverse transcriptase with an IC50 of 0.28 microM and proliferation of both hepatoma HepG2 and breast cancer MCF-7 cells with an IC50 of approx. 2 microM. It induced apoptosis in HepG2 and MCF-7 cells. It manifested a weaker mitogenic activity on mouse splenocytes than ConA (concanavalin A). It inhibited mycelial growth in Valsa mali with an IC50 of 18 microM.
从刺山柑（Capparis spinosa）种子中纯化出一种具有新型N端氨基酸序列的二聚体62 kDa凝集素。纯化方案包括阴离子交换色谱、阳离子交换色谱，最后通过FPLC在Superdex 75上进行凝胶过滤。实现了约100倍的纯化。该凝集素的血凝活性在pH值1 - 12和高达40℃的范围内稳定，在1 mM浓度下可被D(+)半乳糖、α-乳糖、棉子糖和鼠李糖抑制，在25 mM L(+)-阿拉伯糖和100 mM D(+)GlcN（葡糖胺）作用下也可被抑制。该凝集素能有效抑制HIV-1逆转录酶，IC50为0.28 μM，对肝癌HepG2细胞和乳腺癌MCF-7细胞的增殖均有抑制作用，IC50约为2 μM。它可诱导HepG2和MCF-7细胞凋亡。与伴刀豆球蛋白A（ConA）相比，它对小鼠脾细胞的促有丝分裂活性较弱。它对苹果腐烂病菌菌丝生长有抑制作用，IC50为18 μM。
