采用高效液相色谱-紫外检测法测定血浆和支气管肺泡灌洗液中游离厄他培南的含量。
Determination of free ertapenem in plasma and bronchoalveolar lavage by high-performance liquid chromatography with ultraviolet detection.
作者信息
Gordien Jean-Baptiste, Boselli Emmanuel, Fleureau Catherine, Allaouchiche Bernard, Janvier Gérard, Lalaude Olivier, Saux Marie-Claude, Breilh Dominique
机构信息
Clinical Pharmacokinetic Department, Victor Segalen Bordeaux 2 University and Clinical Pharmacy Haut-Lévêque Hospital, Bordeaux, France.
出版信息
J Chromatogr B Analyt Technol Biomed Life Sci. 2006 Jan 18;830(2):218-23. doi: 10.1016/j.jchromb.2005.10.037. Epub 2005 Nov 14.
A sensitive assay for the determination of unbound ertapenem in human plasma and bronchoalveolar lavage (BAL) was developed using ultrafiltration of plasma and BAL samples. A rapid HPLC method was used with ultraviolet detection set at a wavelength of 305 nm and a separation on a Prontosil AQ C18 column, with imipenem used as internal standard. This assay was linear over the concentration range of 0.5-100 microg/mL and 0.25-50 microg/mL in plasma and BAL, respectively. Limits of detection and quantitation were respectively 0.05 and 0.25 microg/mL. Validation data for accuracy and precision were CV<2.48 and 8.25%, accuracy in the range 98.1-104.2% and 102.2-108.4%, respectively, for intra and inter-day.
采用血浆和支气管肺泡灌洗（BAL）样本超滤法，开发了一种灵敏的测定人血浆和BAL中游离厄他培南的方法。使用快速高效液相色谱法，紫外检测波长设定为305nm，在Prontosil AQ C18柱上进行分离，以亚胺培南作为内标。该测定法在血浆和BAL中的线性浓度范围分别为0.5 - 100μg/mL和0.25 - 50μg/mL。检测限和定量限分别为0.05和0.25μg/mL。日内和日间的精密度和准确度验证数据分别为变异系数（CV）<2.48%和8.25%，准确度范围分别为98.1 - 104.2%和102.2 - 108.4%。
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