Faisal Kunnathodi, Faridha Abdul, Akbarsha Mohammad A
Department of Animal Science, School of Life Sciences, Bharathidasan University, Tiruchirappalli 620024, India.
Reprod Toxicol. 2008 Nov-Dec;26(3-4):303-9. doi: 10.1016/j.reprotox.2008.09.003. Epub 2008 Sep 20.
Dietary aflatoxins produce a disease state known as aflatoxicosis, and disruption of spermatogenesis is one of its serious consequences. Towards finding the cellular targets in spermatogenic compartment for aflatoxin toxicity, aflatoxin B(1) (AFB(1)) was administered to 90-day-old Swiss mouse through i.p. route at a daily dose of 20mug per kg body weight for 7, 15, 35 and 45 days. The testis and epididymis were subjected to light- as well as transmission electron microscopic analysis. One of the newer observations was occurrence of meiotic micronucleate giant spermatocytes in seminiferous epithelium and epididymal lumen. The origin of these cells could be traced to imminent disruption of spindle apparatus during meiotic division of spermatocytes, resulting in lagging of chromosome bivalents or replicated univalents. Such chromosomes appeared to undergo condensation and become micronuclei. Thus, this study reports that aflatoxin exposure would result in generation of meiotic micronucleate giant spermatocytes.
膳食中的黄曲霉毒素会引发一种名为黄曲霉毒素中毒的疾病状态，而精子发生过程的紊乱是其严重后果之一。为了找到黄曲霉毒素毒性作用于生精区室的细胞靶点，将黄曲霉毒素B1（AFB1）以每千克体重每日20微克的剂量通过腹腔注射途径给予90日龄的瑞士小鼠，持续7天、15天、35天和45天。对睾丸和附睾进行了光学显微镜以及透射电子显微镜分析。一项较新的观察结果是在生精上皮和附睾管腔中出现了减数分裂微核巨型精母细胞。这些细胞的起源可追溯到精母细胞减数分裂期间纺锤体装置即将发生的破坏，导致染色体二价体或复制的单价体滞后。这样的染色体似乎会发生浓缩并形成微核。因此，本研究报告称，接触黄曲霉毒素会导致减数分裂微核巨型精母细胞的产生。
