Mooers Blaine H M
Department of Biochemistry and Molecular Biology, University of Oklahoma Health Sciences Center, 975 NE 10th Street, BRC 466, Oklahoma City, OK 73104, USA.
Methods. 2009 Mar;47(3):168-76. doi: 10.1016/j.ymeth.2008.09.006. Epub 2008 Oct 10.
Our knowledge of nucleic acid structure grew rapidly over the past decade with the determination to high resolution of larger structures of great biological significance. Advances in sample preparation, crystallization techniques, cryocrystallography, access to synchrotron radiation, and crystallographic software continue to accelerate the structure determination of nucleic acids. Crystallographic studies of DNA and RNA molecules share many considerations that we outline here. The application of crystallography to RNA is illustrated with the structure determination of the CUG repeat that is linked to type I myotonic dystrophy.
在过去十年中，随着具有重大生物学意义的更大结构的高分辨率测定，我们对核酸结构的认识迅速增长。样品制备、结晶技术、低温结晶学、同步辐射的获取以及晶体学软件方面的进展继续加速核酸的结构测定。DNA和RNA分子的晶体学研究有许多共同的考虑因素，我们在此进行概述。通过与I型强直性肌营养不良相关的CUG重复序列的结构测定来说明晶体学在RNA中的应用。
