Anderson A C, Earp B E, Frederick C A
Committee on Higher Degrees in Biophysics, Harvard University and Dana-Farber Cancer Institute, Boston, MA 02115, USA.
J Mol Biol. 1996 Jun 21;259(4):696-703. doi: 10.1006/jmbi.1996.0351.
The determination of RNA structures by X-ray crystallography is an exciting and developing field. At present, the crystallographic characterization of RNA is limited by the difficulty in obtaining large, high quality crystals. This paper outlines several techniques for improving the likelihood of obtaining RNA crystals, for improving the size of those crystals, and for extending the limit of the diffraction maxima. Sequence variations have proven to be more effective in changing the quality of the crystals than variations in crystallization conditions, often making the difference between obtaining true single crystals and multiply twinned crystalline material.
通过X射线晶体学确定RNA结构是一个令人兴奋且不断发展的领域。目前，RNA的晶体学表征受到难以获得大尺寸、高质量晶体的限制。本文概述了几种提高获得RNA晶体的可能性、增大晶体尺寸以及扩展衍射最大值极限的技术。事实证明，序列变异在改变晶体质量方面比结晶条件的变异更有效，这常常决定了能否获得真正的单晶与多重孪晶材料。
