Srivastava M, Gupta S P
Z Parasitenkd. 1977 Jun 3;52(1):61-8. doi: 10.1007/BF00380559.
Adult parasites, Isoparorchis hypselobagri were maintained in vitro in four basic salt solutions, viz. Stephenson saline, AB saline, Tyrode's solution and Locke's solution. Survival was prolonged by adding sugars to Tyrode's solution. Of all the sugars, glucose was found most effective and parasites survived for 39 days in solution containing glucose, 6 days in galactose and sucrose, 10 days in fructose and lactose and 12 days in maltose. The parasites absorb carbohydrates through the cuticle. Ligated parasites survived for a longer period than unligated parasites. The pH 9 was considered as an optimum for maximum survival of the parasites Isoparorchis hypselobagri for 39 days.
成年寄生虫伊氏鲿等睾吸虫在四种基本盐溶液中进行体外培养，即斯蒂芬森生理盐水、AB生理盐水、台氏液和洛克氏液。通过向台氏液中添加糖类可延长其存活时间。在所有糖类中，发现葡萄糖最有效，寄生虫在含葡萄糖的溶液中存活39天，在半乳糖和蔗糖溶液中存活6天，在果糖和乳糖溶液中存活10天，在麦芽糖溶液中存活12天。寄生虫通过角质层吸收碳水化合物。结扎的寄生虫比未结扎的寄生虫存活时间更长。pH 9被认为是伊氏鲿等睾吸虫存活39天的最佳酸碱度。
