Nishikawa Masahiro, Hosokawa Kenta, Ishiguro Mai, Minamioka Hiroki, Tamura Kentaro, Hara-Nishimura Ikuko, Takahashi Yohei, Shimazaki Ken-ichiro, Imai Hiroyuki
Department of Biology, Graduate School of Natural Science, Konan University, Kobe, 658-8501, Japan.
Plant Cell Physiol. 2008 Nov;49(11):1758-63. doi: 10.1093/pcp/pcn149. Epub 2008 Oct 11.
Sphingoid long-chain base (LCB) 1-phosphates are degradated by LCB 1-phosphate lyase to C(16) fatty aldehydes and phosphoethanolamine. Here, we confirmed that the At1g27980 gene product, AtDPL1, is a functional LCB-1-phosphate lyase. Expression of green fluorescent protein fusion products in suspension-cultured Arabidopsis cells showed that AtDPL1 is located to the endoplasmic reticulum. The rates of fresh weight decreases of dpl1-1 and dpl1-2 mutants were significantly slower than those of the wild-type plants. This ability to limit their transpiration reflected the leaf temperature of the mutant plants more than that of wild-type plants, suggesting that AtDPL1 plays a role in dehydration stress.
鞘氨醇长链碱（LCB）1-磷酸酯被LCB 1-磷酸裂解酶降解为C（16）脂肪醛和磷酸乙醇胺。在此，我们证实At1g27980基因产物AtDPL1是一种功能性LCB-1-磷酸裂解酶。绿色荧光蛋白融合产物在悬浮培养的拟南芥细胞中的表达表明AtDPL1定位于内质网。dpl1-1和dpl1-2突变体的鲜重下降速率明显慢于野生型植株。这种限制蒸腾作用的能力使突变体植株的叶片温度比野生型植株更能反映出来，表明AtDPL1在脱水胁迫中起作用。
