Yamasaki S, Shirai H, Takeda Y, Nishibuchi M
Department of Microbiology, Faculty of Medicine, Kyoto University, Japan.
FEMS Microbiol Lett. 1991 May 15;64(2-3):259-63. doi: 10.1016/0378-1097(91)90606-b.
The gene (designated as Vh-tdh) of Vibrio hollisae 9041 encoding a hemolysin similar to the thermostable direct hemolysin (TDH) of V. parahaemolyticus contained a 567-base-pair open reading frame (ORF), which was 93.3-93.5% homologous to those of the tdh genes of V. parahaemolyticus, V. cholerae non-01, and V. mimicus encoding TDH or similar hemolysins. Comparative analysis of the nucleotide sequence containing the Vh-tdh ORF with published nucleotide and amino acid sequences suggested that the Vh-tdh gene and other tdh genes diverged from a common ancestral gene, that the divergence was closely associated with the evolutionary divergence of V. hollisae from other species of genus Vibrio, and that strain-to-strain variation of the Vh-tdh gene exists in V. hollisae.
霍利斯弧菌9041中编码一种与副溶血性弧菌耐热直接溶血素（TDH）相似的溶血素的基因（命名为Vh-tdh）含有一个567个碱基对的开放阅读框（ORF），该开放阅读框与副溶血性弧菌、非O1群霍乱弧菌和模仿弧菌中编码TDH或相似溶血素的tdh基因的同源性为93.3%-93.5%。对包含Vh-tdh开放阅读框的核苷酸序列与已发表的核苷酸和氨基酸序列进行比较分析表明，Vh-tdh基因和其他tdh基因源自一个共同的祖先基因，这种分化与霍利斯弧菌与弧菌属其他物种的进化分化密切相关，并且霍利斯弧菌中存在Vh-tdh基因的菌株间变异。
