Padmini E, Vijaya Geetha B, Usha Rani M
Department of Biochemistry, Bharathi Women's College, Chennai-108, Tamilnadu, India.
Braz J Med Biol Res. 2008 Nov;41(11):951-5. doi: 10.1590/s0100-879x2008005000038. Epub 2008 Oct 3.
The objective of this study was to determine the liver oxidative stress status of grey mullets living in heavy-metal-rich polluted Ennore estuary compared with unpolluted Kovalam estuary. Fish were collected from both estuaries during the monsoon and summer seasons from October 2004 to September 2006. Fish liver homogenate (N = 20 per group) was prepared for evaluating oxidative stress parameters. Fish living in the polluted estuary had significantly higher lipid oxidation products, conjugated dienes (0.346 +/- 0.017 vs 0.141 +/- 0.012 DA233/mg protein), lipid hydroperoxides (0.752 +/- 0.032 vs 0.443 +/- 0.03 nmol/mg protein), and lipid peroxides (3.447 +/- 0.14vs 1.456 +/- 0.096 nmol MDA/mg protein) than those of the unpolluted estuary during the summer. In contrast, significantly lower levels of superoxide dismutase (20.39 +/- 1.14 vs 53.63 +/- 1.48 units/mg protein) and catalase (116 +/- 6.87vs 153 +/- 8.92 units/mg protein) were detected in the liver of fish from the polluted estuary (Ennore) compared to fish from the unpolluted estuary (Kovalam) during the summer. Variations in most of the oxidative stress parameters were observed between the summer and monsoon seasons, indicating the importance of seasonal variation for estuaries and their inhabitants.
本研究的目的是确定生活在重金属污染严重的埃努鲁尔河口的鲻鱼与未受污染的科瓦拉姆河口的鲻鱼相比，其肝脏的氧化应激状态。在2004年10月至2006年9月的季风季节和夏季，从这两个河口采集鱼类。制备鱼肝匀浆（每组N = 20）以评估氧化应激参数。在夏季，生活在污染河口的鱼类的脂质氧化产物、共轭二烯（0.346±0.017 vs 0.141±0.012 DA233/毫克蛋白质）、脂质氢过氧化物（0.752±0.032 vs 0.443±0.03纳摩尔/毫克蛋白质）和脂质过氧化物（3.447±0.14 vs 1.456±0.096纳摩尔丙二醛/毫克蛋白质）显著高于未受污染河口的鱼类。相比之下，在夏季，与未受污染河口（科瓦拉姆）的鱼类相比，污染河口（埃努鲁尔）的鱼类肝脏中超氧化物歧化酶（20.39±1.14 vs 53.63±1.48单位/毫克蛋白质）和过氧化氢酶（116±6.87 vs 153±8.92单位/毫克蛋白质）的水平显著降低。在夏季和季风季节之间观察到大多数氧化应激参数的变化，表明季节变化对河口及其居民的重要性。
