Liu Yi-Hsiang, Cheng Chiung-Chi, Ho Chin-Chin, Chao Wei-Ting, Pei Ren-Jeng, Hsu Yung-Hsiang, Yeh Kun-Tu, Ho Lu-Chang, Tsai Ming-Chuang, Lai Yih-Shyong
Department of Pathology, Jen Ai Hospital, Taichung, Taiwan, R.O.C.
In Vivo. 2008 Sep-Oct;22(5):543-8.
Hepatoma cells are morphologically different from those of the normal liver. Intermediate filaments (IFs) are important in building the cellular architecture and maintaining the outline of cells. Plectin is a cross-linking protein that organizes the cytoskeleton into a stable meshwork, which can maintain the uniform size and shape of hepatocytes. Apoptosis might be the most possible pathway for creating plectin deficiency in the in vivo state.
Apoptosis was induced by staurosporine (STS) treatment in liver cells. The protein expression of cytokeratin 18 (CK18) and plectin as well as the morphology of the liver cells and the distribution of CK18 and plectin in the cells was studied after STS treatment.
Plectin was cleaved in the liver cells during apoptosis and CK18 was modulated. Morphological changes were observed in the liver cells.
By affecting the organization of IFs, plectin might play an important role in the pleomorphism of hepatoma cells and even the tumorigenesis of hepatoma.
肝癌细胞在形态上与正常肝细胞不同。中间丝（IFs）在构建细胞结构和维持细胞外形方面很重要。网蛋白是一种交联蛋白，可将细胞骨架组织成稳定的网络，从而维持肝细胞的均匀大小和形状。凋亡可能是体内造成网蛋白缺乏的最可能途径。
用星形孢菌素（STS）处理肝细胞诱导凋亡。研究STS处理后肝细胞中细胞角蛋白18（CK18）和网蛋白的蛋白表达、肝细胞形态以及CK18和网蛋白在细胞中的分布。
凋亡过程中肝细胞中的网蛋白被切割，CK18受到调节。观察到肝细胞有形态学变化。
通过影响中间丝的组织，网蛋白可能在肝癌细胞的多形性甚至肝癌的肿瘤发生中起重要作用。
