Ling Min, Qin Yongling, Li Nan, Liang Zhiqun
College of Life Science and Technology, Guangxi University, Nanning, 530004, People's Republic of China.
Biotechnol Lett. 2009 Feb;31(2):227-31. doi: 10.1007/s10529-008-9857-4. Epub 2008 Oct 15.
Xyr1 (xylanase regulator 1) and ACEI are two transcriptional factors that regulate xylanases and cellulases formation in Trichoderma reesei. To investigate the transcriptional regulation of cellulases, DNA binding domains of both Xyr1 and ACEI in T. reesei were expressed from E. coli. Electrophoresis mobility shift assays showed that both formed complexes with the cbh1 promoter fragment (-304 to -18), suggesting that Xyr1 and ACEI may regulate cbh1 expression by bindings to the corresponding sites in the cbh1 promoter.
Xyr1（木聚糖酶调节因子1）和ACEI是两种调节里氏木霉中木聚糖酶和纤维素酶形成的转录因子。为了研究纤维素酶的转录调控，在大肠杆菌中表达了里氏木霉中Xyr1和ACEI的DNA结合结构域。电泳迁移率变动分析表明，二者均与cbh1启动子片段（-304至-18）形成复合物，这表明Xyr1和ACEI可能通过与cbh1启动子中的相应位点结合来调节cbh1的表达。
