Guest S, Ngo V V, Hefford M A
Centre for Biologics Research, Biologics and Genetic Therapies Directorate, Health Canada, 251 Sir Frederick Banting Driveway, Ottawa ON, Canada.
Protein Pept Lett. 2008;15(8):811-7. doi: 10.2174/092986608785203809.
Circular dichroism reports that glucagon in solution becomes increasingly helical with the addition of fluoroalcohol (which also decreases solution pH), and with changes in pH and ionic strength. Given the variability of structure observed, these data indicate that care must be taken when comparing results obtained under different solution conditions.
圆二色性表明，随着氟代醇的添加（这也会降低溶液的pH值）以及pH值和离子强度的变化，溶液中的胰高血糖素会变得越来越呈螺旋状。鉴于观察到的结构变异性，这些数据表明，在比较不同溶液条件下获得的结果时必须谨慎。
