Thompson T S, Kolic T M, MacPherson K A
Ministry of the Environment, Dioxin Laboratory, Rexdale, Ontario, Canada.
J Chromatogr. 1991 Apr 26;543(1):49-58. doi: 10.1016/s0021-9673(01)95754-1.
A high-performance liquid chromatographic cleanup procedure employing normal-phase alumina and carbon--silica separations was developed for isolating polychlorinated dibenzo-p-dioxins (PCDDs) and dibenzofurans (PCDFs) from other polychlorinated pollutants present in fish tissue. The method utilizes a column-switching step where the dioxins and furans are trace enriched onto a carbon-silica column as they are eluted from the alumina column. Interfering components such as polychlorinated biphenyls and chlorinated diphenyl ethers elute through the carbon--silica column. The PCDDs and PCDFs are subsequently recovered by backflushing the carbon--silica column using toluene.
开发了一种采用正相氧化铝和碳 - 硅胶分离的高效液相色谱净化程序，用于从鱼组织中存在的其他多氯污染物中分离多氯二苯并 - p - 二恶英（PCDDs）和二苯并呋喃（PCDFs）。该方法利用柱切换步骤，当二恶英和呋喃从氧化铝柱洗脱时，它们会被微量富集到碳 - 硅胶柱上。多氯联苯和氯化二苯醚等干扰成分则通过碳 - 硅胶柱洗脱。随后使用甲苯反冲碳 - 硅胶柱来回收PCDDs和PCDFs。
