Izat A L, McGinnis J, Reiber M A, Kopek J M, Hierholzer R E, Mauromoustakos A
Department of Animal and Poultry Sciences, University of Arkansas, Fayetteville 72701.
Poult Sci. 1991 Jan;70(1):184-7. doi: 10.3382/ps.0700184.
Two trials were conducted to compare the standard culture procedure and the Report Salmonella Visual Immunoassay for detection of salmonellae in rinse fluid recovered from raw poultry carcasses. Both assays were evaluated with and without preenrichment in lactose broth prior to enrichment in selenite cystine broth. Live birds were inoculated via the drinking water prior to processing to ensure a high degree of carcass contamination. Prechill carcasses were sampled using 200 mL of sterile water. Results indicated that lactose preenrichment was not necessary for salmonellae evaluation using standard culture procedures. However, the Report assay produced fewer false-negative results if lactose preenrichment was utilized.
进行了两项试验，以比较标准培养程序和沙门氏菌视觉免疫检测法用于检测从生禽屠体回收的冲洗液中的沙门氏菌。在亚硒酸盐胱氨酸肉汤中富集之前，两种检测方法均在有无乳糖肉汤预富集的情况下进行评估。在加工前通过饮用水对活禽进行接种，以确保屠体受到高度污染。使用200 mL无菌水对预冷后的屠体进行采样。结果表明，使用标准培养程序评估沙门氏菌时，乳糖预富集并非必要。然而，如果采用乳糖预富集，视觉免疫检测法产生的假阴性结果会更少。
