Dorgan Neto V, Saad Júnior R, Hell N S, Bianco A C, Santo G C, Rasslan S
Departamento de Cirurgia, Faculdade de Ciências Médicas, Santa Casa de São Paulo.
Rev Paul Med. 1991 Mar-Apr;109(2):71-6.
A study was made of the changes in the cell population producing gastrin of the gastric antrum in rats submitted to resection of 80% of jejunum-ileum. Ninety days after surgery, the animals were killed after a 12 hour nightly fast and the gastric antrum was removed with the objective of specific histological preparations (PAP method) in order to count the G-cells and the blood was taken for serum doses of gastrin. An optical microscope was used to count the cells using a histometric integraded ocular of 42 points and the counting of 10 fields of each histological cut, and the radioimmunoassay method of double antibody was used for the seric dosing of gastrin. Histometry showed a significant drop in the G-cell population of the antrum of enterectomized animals when compared to the control group. Average percentage of G-cells found were 17.55% in the control group and 7.99% in the enterectomized ones. Blood dosing of hormone showed a significant increase of gastrin in the enterectomized animals when compared to controls. Average value of gastrin dosing the control group was 110 Pg/ml and 170 Pg/ml in enterectomized animals. Therefore, the present study permits to conclude that after resection of 80% of jejunum-ileum, there was a decrease in the G-cell population with gastrin in the gastric antrum even in the presence of increased serum gastrin.
对切除80%空肠-回肠的大鼠胃窦中产生胃泌素的细胞群体变化进行了研究。术后90天，动物在夜间禁食12小时后处死，取出胃窦用于特定的组织学制备（PAP法）以计数G细胞，并采集血液用于测定血清胃泌素剂量。使用光学显微镜，通过具有42个点的组织计量积分目镜对细胞进行计数，对每个组织切片的10个视野进行计数，并使用双抗体放射免疫测定法对血清胃泌素进行定量。组织计量学显示，与对照组相比，肠切除动物胃窦的G细胞群体显著减少。对照组中发现的G细胞平均百分比为17.55%，肠切除动物中为7.99%。激素的血液定量显示，与对照组相比，肠切除动物的胃泌素显著增加。对照组胃泌素定量的平均值为110Pg/ml，肠切除动物为170Pg/ml。因此，本研究可以得出结论，在切除80%空肠-回肠后，即使血清胃泌素增加，胃窦中含胃泌素的G细胞群体也会减少。
