Nooter K, Ghio R, van der Berg K J, Bentvelzen P A
Hamatol Bluttransfus. 1976;19:151-9. doi: 10.1007/978-3-642-87524-3_15.
B.M. cells of RLV-infected BALB/c mice can proliferate in methylcellulose in the absence of E.P., while normal B.M. cells cannot (12). Not only the more primitive BFU-E shows hormone-independency (18). This phenomenon is in favour of the view that the Rauscher virus induced erythroblastosis is a true neoplasia although transplantation experiments failed so far. The experiments in which transformation in vitro of B.M. cells by RLV is established (19) show that the CFU-E can serve as a target for the virus. Treatment of normal mice with CFA leads to a rapid increase in CFU-E in the bone marrow (18). Splenomegaly of RLV-infected mice is enhanced by CFA-treatment probably due to an increase in targets. Transfection with proviral DNA also can transform the CFU-E of BALB-c mice. This approach allows in vitro studies on the resistence of mouse strains to RLV in vitro. The studies are of interest for the human disease in two aspects. In vitro transformation assays are needed to study the oncogenic potential of putative human leukemia viruses. Furthermore the studies have yielded some new insight in the pathogenesis of virally induced erythroblastosis. This might serve as a model for e.g. acute myeloid leukemia in man.
感染RLV的BALB/c小鼠的骨髓细胞在无促红细胞生成素的情况下可在甲基纤维素中增殖，而正常骨髓细胞则不能（12）。不仅更原始的爆式红系集落形成单位表现出激素非依赖性（18）。这一现象支持劳斯氏病毒诱导的成红细胞增多症是一种真正的肿瘤形成的观点，尽管到目前为止移植实验失败了。建立RLV对骨髓细胞进行体外转化的实验（19）表明，红系集落形成单位可作为病毒的靶标。用完全弗氏佐剂处理正常小鼠会导致骨髓中红系集落形成单位迅速增加（18）。完全弗氏佐剂处理会增强感染RLV小鼠的脾肿大，这可能是由于靶标的增加。用前病毒DNA转染也可转化BALB - c小鼠的红系集落形成单位。这种方法允许在体外研究小鼠品系对RLV的抗性。这些研究在两个方面对人类疾病具有重要意义。需要进行体外转化试验来研究假定的人类白血病病毒的致癌潜力。此外，这些研究在病毒诱导的成红细胞增多症的发病机制方面有了一些新的见解。这可能为人的急性髓系白血病等疾病提供一个模型。
