Iwasaki S, Wanichanon C
Department of Anatomy, School of Dentistry at Niigata, Nippon Dental University, Japan.
Tissue Cell. 1991;23(3):385-91. doi: 10.1016/0040-8166(91)90056-y.
Scanning and transmission electron microscopy was employed to investigate the ultrastructure of the lingual dorsal epithelial cells of the frog, Rana rugosa. The specimens for scanning electron microscopy were prepared by a method that involved osmium postfixation and treatment with acid to remove extracellular material that adhered to the surface of the tongue. Over almost the entire dorsal surface, filiform papillae, consisting of a large number of non-ciliated cells with microridges and a very small number of ciliated cells, were compactly distributed. Fungiform papillae were scattered among these filiform papillae. A round sensory disk was located on the top of each fungiform papilla. Each sensory disk was encircled by a band of ciliated cells. Transmission electron microscopy revealed that a large part of the filiform papillar epithelium was composed of cells that contained numerous electron-dense granules. These cells were coincident with the non-ciliated cells observed by scanning electron microscopy. In these cells, the nucleus was located on the basal side, and the ergastoplasm was well-developed on the basal side of the nucleus.
采用扫描电子显微镜和透射电子显微镜研究了虎纹蛙舌背上皮细胞的超微结构。扫描电子显微镜标本的制备方法包括锇后固定以及用酸处理以去除粘附在舌表面的细胞外物质。在几乎整个背表面上，丝状乳头紧密分布，其由大量具有微嵴的无纤毛细胞和极少数纤毛细胞组成。菌状乳头散在于这些丝状乳头之间。每个菌状乳头顶部有一个圆形感觉盘。每个感觉盘被一圈纤毛细胞环绕。透射电子显微镜显示，丝状乳头上皮的大部分由含有大量电子致密颗粒的细胞组成。这些细胞与扫描电子显微镜观察到的无纤毛细胞一致。在这些细胞中，细胞核位于基侧，内质网在细胞核基侧发育良好。
