Ultrastructural study of the dorsal lingual epithelium of the soft-shell turtle, Trionyx cartilagineus (Chelonia, Trionychidae).

BACKGROUND

The soft-shell turtle, Trionyx cartilagineus, is classified phylogenetically to the family Trionychidae, whose members live in small rivers or ponds. The purpose of the present study was to examine the ultrastructure of the dorsal epithelium of the tongue of the soft-shell turtle and to compare the results of the observations with those reported for the tongue of other freshwater turtles.

METHODS

Light microscopy, transmission electron microscopy, and scanning electron microscopy were used to examine the dorsal epithelium of the tongue of the soft-shell turtle.

RESULTS

The tongue is triangular with a slightly round apex when viewed dorsally but it appears flattened when viewed laterally. Lingual papillae were visible on the dorsal surface of the tongue with some localized variations. Irregular, dome-shaped or ridge-like papillae were observed on the anterior part of the dorsal lingual surface. Large, cylindrical papillae were located along the midline of the posterior part of the tongue. Low, disk-like papillae were located on both sides of the dorsal surface of the posterior part of the tongue. Taste pores were recognizable in the center of the disk-like papillae. At higher magnification, scanning electron microscopy revealed microridges on the surface of cells located on the outermost side of the anterior part of the tongue, and the thickenings of cell margins were clearly seen. Microvilli were distributed compactly over the entire posterior part of the tongue. Light microscopy revealed that the mucosal epithelium of the anterior part of the tongue was of the keratinized, stratified squamous type, while the mucosal epithelium of the posterior part of the tongue was of the nonkeratinized, stratified cuboidal type. In the latero-posterior part of the tongue, taste buds were recognized. Transmission electron microscopy revealed that the epithelium of the anterior part of the tongue was of a typical keratinized type. Small numbers of keratohyalin granules and membrane-coating granules appeared in the cytoplasm of the shallow intermediate layer. On the apical side of the lingual papillae located on the posterior side of the tongue, cells from the intermediate layer to the surface layer of the non-keratinized epithelium contained many fine, discoidal granules. A large part of the epithelium consisted of mucous cells in the concave area on the posterior side.

CONCLUSIONS

The dorsal surface and epithelium of the tongue of the soft-shell turtle differed significantly from those of other freshwater turtles, in spite of the similarity in terms of gross morphology among the tongues of such turtles.