兔出血症病毒的基因组RNA和亚基因组RNA均与蛋白质相连并包装进病毒粒子中。
Genomic and subgenomic RNAs of rabbit hemorrhagic disease virus are both protein-linked and packaged into particles.
作者信息
Meyers G, Wirblich C, Thiel H J
机构信息
Federal Research Centre for Virus Diseases of Animals, Tübingen, Federal Republic of Germany.
出版信息
Virology. 1991 Oct;184(2):677-86. doi: 10.1016/0042-6822(91)90437-g.
Abstract
The major subgenomic RNA of the calicivirus rabbit hemorrhagic disease virus which codes for the viral capsid protein has been cloned as cDNA. The nucleotide sequence of this mRNA was shown to be identical to the 3' terminal region of the genomic RNA. The 5' end of the mRNA corresponds to position 5296 of the genomic sequence; except for two differences the first 16 nucleotides of genomic and subgenomic RNAs are identical. After isolation from liver tissue viral genomic and subgenomic RNAs were found to be resistant to RNase degradation. This protection was due to RNA packaging into particles. Sucrose density gradient centrifugation of liver homogenates allowed separation of such particles containing either genomic RNA or subgenomic RNA. Genomic and subgenomic RNAs are protein-linked and for the genomic molecule this interaction is localized within the first 179 nucleotides. After radioactive labeling of purified RNA and subsequent RNase treatment a protein of 15 kDa was identified.
摘要
杯状病毒兔出血症病毒编码病毒衣壳蛋白的主要亚基因组RNA已被克隆为cDNA。该mRNA的核苷酸序列与基因组RNA的3'末端区域相同。mRNA的5'端对应于基因组序列的第5296位；除了两个差异外，基因组RNA和亚基因组RNA的前16个核苷酸是相同的。从肝组织中分离后，发现病毒基因组RNA和亚基因组RNA对RNase降解具有抗性。这种保护作用是由于RNA包装成颗粒。肝匀浆的蔗糖密度梯度离心可分离出含有基因组RNA或亚基因组RNA的颗粒。基因组RNA和亚基因组RNA与蛋白质相连，对于基因组分子，这种相互作用位于前179个核苷酸内。对纯化的RNA进行放射性标记并随后进行RNase处理后，鉴定出一种15 kDa的蛋白质。
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本文引用的文献
1
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
2
A protein, VPg, covalently linked to 36S calicivirus RNA.
J Gen Virol. 1980 Mar;47(1):215-20. doi: 10.1099/0022-1317-47-1-215.
3
Characterization of leader RNA sequences on the virion and mRNAs of mouse hepatitis virus, a cytoplasmic RNA virus.小鼠肝炎病毒（一种细胞质RNA病毒）病毒粒子和信使核糖核酸上前导RNA序列的特征分析
Proc Natl Acad Sci U S A. 1984 Jun;81(12):3626-30. doi: 10.1073/pnas.81.12.3626.
4
Self-splicing RNA: autoexcision and autocyclization of the ribosomal RNA intervening sequence of Tetrahymena.自我剪接RNA：嗜热四膜虫核糖体RNA间隔序列的自动切除与自动环化
Cell. 1982 Nov;31(1):147-57. doi: 10.1016/0092-8674(82)90414-7.
5
Spliced and unspliced messenger RNAs synthesized from cloned influenza virus M DNA in an SV40 vector: expression of the influenza virus membrane protein (M1).从SV40载体中克隆的流感病毒M DNA合成的剪接和未剪接信使RNA：流感病毒膜蛋白（M1）的表达
Virology. 1982 Dec;123(2):237-56. doi: 10.1016/0042-6822(82)90258-6.
6
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.在噬菌体T4头部组装过程中结构蛋白的切割
Nature. 1970 Aug 15;227(5259):680-5. doi: 10.1038/227680a0.
7
A one tube reaction for the synthesis of blunt-ended double-stranded cDNA.一种用于合成平端双链 cDNA 的单管反应。
Nucleic Acids Res. 1988 Mar 25;16(6):2726. doi: 10.1093/nar/16.6.2726.
8
An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs.对699种脊椎动物信使核糖核酸的5'-非编码序列的分析。
Nucleic Acids Res. 1987 Oct 26;15(20):8125-48. doi: 10.1093/nar/15.20.8125.
9
Further characterization of the virus-specific RNAs in feline calicivirus infected cells.猫杯状病毒感染细胞中病毒特异性RNA的进一步特性分析。
Virus Res. 1988 Aug;11(1):59-72. doi: 10.1016/0168-1702(88)90067-6.
10
Coronaviruses: structure and genome expression.冠状病毒：结构与基因组表达
J Gen Virol. 1988 Dec;69 ( Pt 12):2939-52. doi: 10.1099/0022-1317-69-12-2939.
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