Kammann M, Schalk H J, Matzeit V, Schaefer S, Schell J, Gronenborn B
Max-Planck-Institut für Züchtungsforschung, Köln, FRG.
Virology. 1991 Oct;184(2):786-90. doi: 10.1016/0042-6822(91)90453-i.
Replication of the single-stranded DNA genome of wheat dwarf virus (WDV) leads to the accumulation of covalently closed double-stranded DNA of genome length in infected cells. By studying the replication properties of a naturally occurring deletion mutant of WDV isolated from infected plants and of deletion mutants constructed in vitro, we have defined cis-acting regions required for viral DNA replication. The results show that two distinct regions are required in cis to yield the normal replicative forms of WDV-DNA.
小麦矮缩病毒（WDV）单链DNA基因组的复制导致受感染细胞中积累基因组长度的共价闭合双链DNA。通过研究从受感染植物中分离出的WDV天然缺失突变体以及体外构建的缺失突变体的复制特性，我们确定了病毒DNA复制所需的顺式作用区域。结果表明，顺式作用需要两个不同区域才能产生WDV-DNA的正常复制形式。
