Andersen M T, Richardson K A, Harbison S A, Morris B A
Plant Diseases Division, DSIR, Auckland, New Zealand.
Virology. 1988 Jun;164(2):443-9. doi: 10.1016/0042-6822(88)90558-2.
The genome of chloris striate mosaic virus (CSMV) comprises a single circular DNA as determined by analyses on virion single-stranded (ss) DNA and virus-specific covalently closed circular (ccc) DNA isolated from infected plants. The nucleotide sequence of CSMV DNA was determined from cccDNA and the data were accommodated into one DNA circle of 2750 nucleotides. Comparison of the nucleotide sequence with those of maize streak virus (MSV), wheat dwarf virus (WDV), and digitaria streak virus (DSV) showed 49, 47, and 48% DNA homology, respectively. The sequence has four potential open reading frames for proteins of greater than 10,000 mol wt, two in the viral (+) sense and two in the complementary (-) sense. Three of these potential coding regions have homologous counterparts, by comparison of the amino acid sequences, among the open reading frames reported for MSV, WDV, and DSV. CSMV encapasidates primer molecules able to prime the synthesis in vitro of a complementary strand to virion DNA, initiating this reaction at one site on the genome. The CSMV primer comprising approximately 88 nucleotides was located within the smaller of two intergenic or noncoding regions.
通过对从受感染植物中分离出的病毒粒子单链（ss）DNA和病毒特异性共价闭合环状（ccc）DNA进行分析确定，条纹虎尾草花叶病毒（CSMV）的基因组由单个环状DNA组成。从cccDNA确定了CSMV DNA的核苷酸序列，并将数据整理到一个2750个核苷酸的DNA环中。将该核苷酸序列与玉米条纹病毒（MSV）、小麦矮缩病毒（WDV）和马唐草条纹病毒（DSV）的核苷酸序列进行比较，分别显示出49%、47%和48%的DNA同源性。该序列有四个潜在的开放阅读框，用于编码分子量大于10,000的蛋白质，其中两个在病毒（+）链上，两个在互补（-）链上。通过比较氨基酸序列，这些潜在编码区域中的三个在MSV、WDV和DSV报道的开放阅读框中有同源对应物。CSMV包裹着能够在体外引发合成与病毒粒子DNA互补链的引物分子，在基因组的一个位点启动该反应。包含约88个核苷酸的CSMV引物位于两个基因间隔区或非编码区中较小的区域内。
