Bácsy E, Tougard C, Tixier-Vidal A, Marton J, Stark E
Histochemistry. 1976;50(2):161-74. doi: 10.1007/BF00495826.
Monolayer cultures of trypsin-dispersed cells of the rat adenohypophysis were grown for 5 to 54 days. ACTH was localized by immunocytochemistry using an antiserum to synthetic ACTH1-28 prepared in rabbit and sheep anti-goat immunoglobulin coupled with peroxidase. ACTH content of the culture medium was measured by radioimmunoassay. Corttion time. The corticotrophs retained their essential morphological characteristics. Immunological staining was found in the secretory granules, some tubular or saccular structures, parts of the rough endoplasmic reticulum, and the cytoplasmic matrix. Immature secretory granules in the Golgi apparatus as well as some Golgi elements showed different degrees of immunoreactivity. In agreement with the high ACTH content of the culture medium the number, size and shape of the secretory granules, the active Golgi apparatus, the high amount of extragranular ACTH as well as pictures suggesting granule extrusion claim for a high ACTH synthesis and transport (and low ACTH storage) in the cultured corticotrophs.
将大鼠腺垂体胰蛋白酶分散细胞进行单层培养5至54天。使用兔抗合成促肾上腺皮质激素1 - 28抗血清以及与过氧化物酶偶联的羊抗山羊免疫球蛋白，通过免疫细胞化学方法定位促肾上腺皮质激素（ACTH）。采用放射免疫分析法测定培养基中ACTH的含量。促肾上腺皮质激素细胞保留了其基本形态特征。在分泌颗粒、一些管状或囊状结构、部分粗面内质网以及细胞质基质中发现免疫染色。高尔基体中的未成熟分泌颗粒以及一些高尔基体成分显示出不同程度的免疫反应性。与培养基中高ACTH含量一致，分泌颗粒的数量、大小和形状、活跃的高尔基体、大量的颗粒外ACTH以及提示颗粒外排的图像表明培养的促肾上腺皮质激素细胞中ACTH合成和运输能力强（而ACTH储存能力低）。
