大鼠垂体前叶细胞的促肾上腺皮质激素放射免疫细胞化学（RICH）
ACTH radioimmunocytochemistry (RICH) on rat anterior pituitary cells.
作者信息
Rappay G, Kárteszi M, Makara G B
出版信息
Histochemistry. 1979 Jan 22;59(3):207-13. doi: 10.1007/BF00495668.
PMID:218911
Abstract
Radioimmunocytochemistry (RICH) was applied to detect corticotrophs in adult rat pituitaries and 8-day-old anterior pituitary monolayers by incubating sections and cultures with 125I-ACTH-anti ACTH immune complexes. After incubations autoradiography was made. In comparison, "conventional" immunostaining was carried out on adjacent sections and parallel cultures. It has been established that RICH is suitable for detection of corticotrophs.
摘要
采用放射免疫细胞化学（RICH）方法，通过用¹²⁵I-促肾上腺皮质激素-抗促肾上腺皮质激素免疫复合物孵育切片和培养物，来检测成年大鼠垂体和8日龄垂体前叶单层培养物中的促肾上腺皮质激素细胞。孵育后进行放射自显影。作为对照，对相邻切片和平行培养物进行“常规”免疫染色。现已证实，RICH适用于促肾上腺皮质激素细胞的检测。
Zotero 插件