高效液相色谱法结合荧光检测测定大鼠脑中的1,2,3,4-四氢异喹啉

High-performance liquid chromatographic determination of 1,2,3,4-tetrahydroisoquinoline in rat brain with fluorescence detection.

作者信息

Ishida J, Yamaguchi M, Nakamura M

机构信息

Faculty of Pharmaceutical Sciences, Fukuoka University, Japan.

出版信息

Anal Biochem. 1991 May 15;195(1):168-71. doi: 10.1016/0003-2697(91)90313-i.

DOI:10.1016/0003-2697(91)90313-i

PMID:1888014

Abstract

A high-performance liquid chromatographic method for the fluorometric determination of 1,2,3,4-tetrahydroisoquinoline in rat brain is described. 1,2,3,4-Tetrahydroisoquinoline and 4-phenylpiperidine (internal standard) are isolated by liquid-liquid extraction, and then converted into the corresponding fluorescent derivatives with 3,4-dihydro-6,7-dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride, a fluorescence derivatization reagent for amines. The derivatives are separated within 60 min on a reversed-phase column, TSK gel ODS-120T, with isocratic elution, and detected fluorometrically. The detection limit of 1,2,3,4-tetrahydroisoquinoline is 1.0 pmol/g in rat brain (S/N = 3).

摘要

本文描述了一种用于荧光测定大鼠脑中1,2,3,4-四氢异喹啉的高效液相色谱方法。1,2,3,4-四氢异喹啉和4-苯基哌啶（内标）通过液-液萃取分离，然后用3,4-二氢-6,7-二甲氧基-4-甲基-3-氧代喹喔啉-2-羰基氯（一种胺类荧光衍生试剂）转化为相应的荧光衍生物。衍生物在反相柱TSK凝胶ODS-120T上通过等度洗脱在60分钟内分离，并进行荧光检测。大鼠脑中1,2,3,4-四氢异喹啉的检测限为1.0 pmol/g（信噪比 = 3）。