Buckwitz D, Jacobasch G, Gerth C
Institut für Biochemie, Humboldt-Universität zu Berlin, Bereich Medizin (Charité), FRG.
Angew Parasitol. 1991 May;32(2):79-85.
A procedure is described for the elucidation of the glycolytic flux rate of red blood cells infected with the malarial parasite Plasmodium berghei. It is based on the titration of the protons originating from the glycolytic lactate accumulation. Compared with traditional methods of biochemical measurements of glucose consumption or accumulation of lactate the proposed procedure shows the following advantages: continuously measurement is possible; constancy of the pH-value during the measurement; lower amounts of biological material are necessary (increased sensitivity) increased accuracy; The method can also be applied to studies on other cells and species with normal or increased glycolytic flux rate.
本文描述了一种用于阐明感染疟原虫伯氏疟原虫的红细胞糖酵解通量率的方法。该方法基于对糖酵解乳酸积累产生的质子进行滴定。与传统的测量葡萄糖消耗或乳酸积累的生化方法相比，该方法具有以下优点：可以进行连续测量；测量过程中pH值恒定；所需生物材料量较少（灵敏度提高），准确性提高；该方法还可应用于对其他具有正常或增加糖酵解通量率的细胞和物种的研究。
