Grinberg L N, Griger M, Iakobash G, Soprunov F F, Miuller M
Vopr Med Khim. 1983 Sep-Oct;29(5):87-93.
Mice erythrocytes, infected with Plasmodium berghei, were studied as a model of integrated cell system "parasite-host erythrocytes". The rate of blood oxygenation, content of methhemoglobin and methhemoglobin reductase activity maintained at the initial level up to the lethal outcome of the infectious process. A steady-state kinetics of glycolysis was studied in the impaired erythrocytes by means of automatic potentiometric titration of lactic acid during incubation of the cells. Distinct 15-20-fold activation of glycolysis occurred as the parasite grew and developed in erythrocytes. Stationary concentration of ATP was decreased at the moment of extensive P. berghei development, accompanied by accumulation in blood of the most mature forms of parasite. At the same time, this step of infection was characterized by a 2-fold increase in content of reduced glutathione and by appearance of of lipoxygenase in the impaired erythrocytes. Activities of superoxide dismutase as well of oxidative pentosephosphate shunt were kept at the initial level. The data obtained are discussed on the basis of current concepts on biochemistry of blood forms of malarial parasite.
以感染伯氏疟原虫的小鼠红细胞作为“寄生虫-宿主红细胞”整合细胞系统的模型进行研究。在感染过程导致小鼠死亡之前，血液氧合速率、高铁血红蛋白含量和高铁血红蛋白还原酶活性均维持在初始水平。通过在细胞孵育期间对乳酸进行自动电位滴定，研究了受损红细胞中糖酵解的稳态动力学。随着寄生虫在红细胞中生长和发育，糖酵解出现明显的15 - 20倍激活。在伯氏疟原虫大量发育时，ATP的稳态浓度降低，同时血液中最成熟形式的寄生虫积累。与此同时，这一感染阶段的特征是受损红细胞中还原型谷胱甘肽含量增加2倍以及出现脂氧合酶。超氧化物歧化酶以及氧化戊糖磷酸途径的活性保持在初始水平。根据目前关于疟原虫血液形式生物化学的概念对所获得的数据进行了讨论。
