Kletzien R F, Perdue J F
J Cell Physiol. 1976 Dec;89(4):723-8. doi: 10.1002/jcp.1040890432.
The mode of induction of sugar transport by serum-stimulation of growth and hexose-starvation in chick embryo fibroblasts (CEF) has been studied using metabolic inhibitors. We have concluded from these studies that the sugar transport increases induced by serum-stimulation are regulated by post-transcriptional mechanisms while sugar transport increases induced by hexose-starvation are regulated by a transcriptional mechanism. CEF infected with a temperature-sensitive mutant of the Rous sarcoma virus. Ts68 and incubated at the nonpermissive temperature for transformation, 41 degrees, retain the capacity to regulate sugar transport in a manner similar to uninfected CEF. However, Ts68-infected CEF maintained at the permissive temperature for transformation, 37 degrees, have lost the ability to regulate sugar transport at the post-transcriptional and post-translational levels.
利用代谢抑制剂研究了血清刺激生长和己糖饥饿诱导鸡胚成纤维细胞（CEF）中糖转运的模式。我们从这些研究中得出结论，血清刺激诱导的糖转运增加是由转录后机制调节的，而己糖饥饿诱导的糖转运增加是由转录机制调节的。用劳斯肉瘤病毒的温度敏感突变体Ts68感染CEF，并在41℃的非允许温度下培养以进行转化，其仍保留以类似于未感染CEF的方式调节糖转运的能力。然而，在允许转化的温度37℃下维持的Ts68感染的CEF，已失去在转录后和翻译后水平调节糖转运的能力。
