狂犬病特异性抗体的定量。I. 改良对流免疫电泳。一种快速灵敏的方法。

The quantitation of rabies-specific antibodies. I. Modified counter immunoelectrophoresis. A rapid and sensitive method.

作者信息

Chauhan A, Sood D K, Saha S M, Aggarwal R K, Kapoor M, Saxena S N

机构信息

Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

Biologicals. 1991 Apr;19(2):93-5. doi: 10.1016/1045-1056(91)90005-5.

DOI:10.1016/1045-1056(91)90005-5

PMID:1888500

Abstract

Modified counter immunoelectrophoresis was standardized with respect to dilution of tissue culture antigen and indicator serum, the incubation time for neutralization and the effect of an electric current. The technique was found to be sensitive enough to detect a minimum level of antibodies (0.5 IU/ml) by using a 16 mA current per slide for 2 h, indicator serum of 15 IU/ml and the use of an antigen at a concentration of 1:35. Above all, the incubation period did not affect the neutralization of the virus. The test was also applied to the detection of rabies-specific antibody levels in 73 human sera. The test was found to be simple, quick and economical for titration of rabies antibodies.

摘要

改良对流免疫电泳在组织培养抗原和指示血清的稀释、中和孵育时间以及电流影响方面进行了标准化。结果发现，该技术足够灵敏，通过每张玻片使用16毫安电流2小时、15国际单位/毫升的指示血清以及使用浓度为1:35的抗原，能够检测到最低水平的抗体（0.5国际单位/毫升）。最重要的是，孵育期不影响病毒的中和。该试验还应用于检测73份人血清中的狂犬病特异性抗体水平。结果发现，该试验用于狂犬病抗体滴定简单、快速且经济。