狂犬病特异性抗体的定量分析。III. 人血清改良对流免疫电泳、血凝抑制及血清中和效价的比较评估

The quantitation of rabies-specific antibodies. III. A comparative evaluation of modified counter immunoelectrophoresis, haemagglutination inhibition and serum neutralization titres of human sera.

作者信息

Chauhan A, Sood D K, Saha S M, Kapoor M, Aggarwal R K, Saxena S N

机构信息

Department of Hepatology, Postgraduate Institute of Medical Education and Research, Chandigarh, India.

出版信息

Biologicals. 1991 Apr;19(2):103-6. doi: 10.1016/1045-1056(91)90007-7.

DOI:10.1016/1045-1056(91)90007-7

PMID:1888488

Abstract

The rabies-specific antibodies of 73 serum samples from vaccinated humans were determined by the modified counter immunoelectrophoresis (MCIE), and the haemagglutination inhibition test (HAI) by using the conventional serum neutralization test (SN) as a yard-stick. Both MCIE and HAI were found to be sensitive and specific for the estimation of rabies antibodies. In general, the unitages obtained by the MCIE and SN showed statistically insignificant differences (P greater than 0.05) and the correlation coefficient between the two methods was 0.697 (P less than 0.05). Although the unitage of the sera detected by HAI tests was lower by a factor of 0.155 from the unitage of SN tests, there was statistically insignificant differences between the two techniques (P greater than 0.05) with a correlation coefficient of 0.556 (P less than 0.05).

摘要

采用改良对流免疫电泳（MCIE）检测了73份接种疫苗的人血清样本中的狂犬病特异性抗体，并以传统血清中和试验（SN）为标准进行血凝抑制试验（HAI）。结果发现，MCIE和HAI用于估计狂犬病抗体时均具有敏感性和特异性。总体而言，MCIE和SN获得的效价显示出无统计学意义的差异（P大于0.05），两种方法之间的相关系数为0.697（P小于0.05）。尽管HAI检测的血清效价比SN检测的效价低0.155倍，但两种技术之间无统计学意义的差异（P大于0.05），相关系数为0.556（P小于0.05）。