NcNab W B, Meek A H, Duncan J R, Martin S W, Van Dreumel A A
Animal Diseases Research Institute, Agriculture Canada, Ontario.
Can J Vet Res. 1991 Jul;55(3):246-51.
An observational study involving 304 dairy herds and three abattoirs was conducted between 1986 and 1989 to investigate the epidemiology of paratuberculosis in dairy cattle in Ontario. The objectives of this paper were to describe the method of data collection for the study, to present descriptive production statistics and to present estimates of the prevalence of paratuberculosis among dairy cattle in Ontario. Mycobacterium paratuberculosis was isolated from the distal ileum and/or the ileocecal lymph node of 5.5% of 400 cull cows. Based on a lipoarabinomannan antigen enzyme-linked immunosorbent assay (LAM-ELISA) on sera, the predicted true prevalence of paratuberculosis among 14,923 dairy cattle from 304 herds, was 6.1%. Nineteen percent of 2,943 fecal cultures were uninterpretable because of overgrowth with contaminating bacteria or fungi. It was concluded that the true prevalence of paratuberculosis among dairy cattle in Ontario was no greater, and may be less than the true prevalence among dairy cattle from various regions of the United States. However, at a practical level, for the purposes of trade policy, the present study suggests that the functional prevalence is very similar in the two countries.
1986年至1989年间，开展了一项涉及304个奶牛场和3个屠宰场的观察性研究，以调查安大略省奶牛副结核病的流行病学情况。本文的目的是描述该研究的数据收集方法，呈现描述性生产统计数据，并给出安大略省奶牛副结核病患病率的估计值。从400头淘汰奶牛的回肠末端和/或回盲部淋巴结中分离出副结核分枝杆菌的比例为5.5%。基于血清中的脂阿拉伯甘露聚糖抗原酶联免疫吸附测定（LAM-ELISA），304个牛群中14923头奶牛的副结核病预测真实患病率为6.1%。2943份粪便培养物中有19%因被污染细菌或真菌过度生长而无法解读。得出的结论是，安大略省奶牛副结核病的真实患病率并不高于，可能还低于美国不同地区奶牛的真实患病率。然而，从实际层面来看，就贸易政策而言，本研究表明两国的实际患病率非常相似。
