Jepras R I, Rarity J, Perkins E A, Clarke D J, Carr R J, Atkinson T
Division of Biotechnology, P.H.L.S. Centre for Applied Microbiology and Research, Porton Down, Wilts, U.K.
Biochem Soc Trans. 1991 Apr;19(2):513-4. doi: 10.1042/bst0190513.
Following a recent successful application of p.c.s. to liquid chromatography in the biotechnology industry, its usefulness as a contamination monitor in the fermentation industry was assessed. It was found that: (i) the intensity bias of the technique limits its uses to the detection of contaminants when they are larger than the host; (ii) the inherent heterogeneity of microbial cultures prevents the use of multiangle studies, and (iii) the large size of bacteria make the use of p.c.s. in flowing, on-line systems impractical.
继最近将多角度光散射（p.c.s.）成功应用于生物技术行业的液相色谱之后，评估了其作为发酵行业污染监测器的实用性。结果发现：（i）该技术的强度偏差限制了其仅用于检测比宿主大的污染物；（ii）微生物培养物固有的异质性使得无法使用多角度研究，并且（iii）细菌的大尺寸使得在流动的在线系统中使用多角度光散射不切实际。
