Billett F S, Adam E
J Embryol Exp Morphol. 1976 Dec;36(3):697-710.
The ultrastructure of the mitochondrial cloud (Balbiani body) of the pre-vitellogenic oocytes of Xenopus laevis has been examined using transmission and stereoscan electron microscopy. Examination of conventional thin sections confirm previous observations which suggest that the cloud consists essentially of many thousands mitochondria and numerous small vesicles; larger clouds, in oocytes greater than 200 mum in diameter, contain relatively more vesicles. Using a standard electron microscope at 100 kV very long and coursing arrays of mitochondrial profiles can be detected. The presence of very long mitochondrial elements has been confirmed using a high voltage microscope operating at 500-1000 kV. Stereoscan preparations, isolated from pre-vitellogenic oocytes, lend some support to the view that the mitochondrial cloud amy consist of a mass of long filamentous mitochondria and the possibility that there are large continuous regions of mitochondrial material cannot be ruled out.
利用透射电子显微镜和立体扫描电子显微镜对非洲爪蟾卵黄发生前卵母细胞的线粒体云（巴尔比亚尼体）的超微结构进行了研究。对常规薄片的检查证实了先前的观察结果，即线粒体云主要由数千个线粒体和众多小泡组成；在直径大于200μm的卵母细胞中，较大的线粒体云含有相对更多的小泡。在100 kV的标准电子显微镜下，可以检测到非常长且呈束状排列的线粒体轮廓。使用500 - 1000 kV的高压显微镜证实了非常长的线粒体元件的存在。从卵黄发生前卵母细胞中分离出的立体扫描标本，为线粒体云可能由大量长丝状线粒体组成的观点提供了一些支持，并且不能排除存在大片连续线粒体物质区域的可能性。
