脑心肌炎病毒RNA的氨酰化作用
Aminoacylation of encephalomyocarditis virus RNA.
作者信息
Lindley I G, Stebbing N
出版信息
J Gen Virol. 1977 Jan;34(1):177-82. doi: 10.1099/0022-1317-34-1-177.
PMID:188978
Abstract
RNA extracted from purified encephalomyocarditis (EMC) virus (EMC-RNA) can be aminoacylated with synthetase praparations from Escherichia coli, beef and rabbit liver. The extent of aminoacylation is between 0-024 and 0-080 moles per mole EMC-RNA and occurs only with serine. Either removal of possible low mol. wt. contaminants with 3 M-sodium acetate nor periodate oxidation of the virus RNA affects its aminoacylation capacity.
摘要
从纯化的脑心肌炎（EMC）病毒中提取的RNA（EMC-RNA）可用来自大肠杆菌、牛肉和兔肝的合成酶制剂进行氨酰化。氨酰化程度为每摩尔EMC-RNA 0.024至0.080摩尔，且仅与丝氨酸发生反应。用3M醋酸钠去除可能的低分子量污染物或对病毒RNA进行高碘酸盐氧化，均不影响其氨酰化能力。
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