Hall T C, Shih D S, Kaesberg P
Biochem J. 1972 Oct;129(4):969-76. doi: 10.1042/bj1290969.
Incubation of tyrosine with the RNA from brome-mosaic-virus under conditions favourable for aminoacylation of tRNA resulted in binding of tyrosine to the viral RNA. Sucrose-density-gradient centrifugation showed that tyrosine binds to three and probably to all four of the viral RNA components. The bound amino acid was readily released in mild alkaline solutions. Of 20 amino acids tested, only tyrosine was found to bind. The maximum extent of binding observed was 0.58mol of tyrosine/mol of brome-mosaic-virus RNA.
在有利于tRNA氨基酰化的条件下,将酪氨酸与来自雀麦花叶病毒的RNA一起温育,结果酪氨酸与病毒RNA结合。蔗糖密度梯度离心表明,酪氨酸与病毒RNA的三个组分结合,可能还与所有四个组分结合。结合的氨基酸在温和的碱性溶液中很容易释放出来。在所测试的20种氨基酸中,仅发现酪氨酸能结合。观察到的最大结合程度为每摩尔雀麦花叶病毒RNA结合0.58摩尔酪氨酸。