Hayano M, Sung J H, Song C W, Clement J J
J Neuropathol Exp Neurol. 1977 Jan;36(1):62-73. doi: 10.1097/00005072-197701000-00007.
Transplantable murine neuroblastoma C 1300 was studied ultrastructurally at varying time intervals ranging from 2 hours to 40 days before and after X-irradiation. Following X-irradiation, 2000 and 4000 rads in a single dose, the uniformly small tumor cells became progressively enlarged multinucleated and degenerated, starting at one to two days. At five to seven days, the uni- and multinucleated giant cells predominated over the small tumor cells, while the giant cells progressively disappeared therafter and the small tumor cells predominated over the giant cells at 10 to 14 days. The giant cells contained abundant subcellular organelles and the X-irradiated tumor cells apparently continued to produce the organelles until they degenerated. Two types of cytoplasmic particles, intracisternal A and bar-shaped, were observed in the tumor cells. The intracisternal A particles occurred in almost all non-irradiated tumor cells though their number varied considerably from cell to cell, while they were observed less frequently in the radiation-induced giant cells probably due to a dilution effect rather than an actual numerical decrease. The bar-shaped particles, hitherto undescribed in the neuroblastoma, were 23 nm in diameter, variable in length and occasionally tubular. They occurred only in degenerating cells regardless of X-irradiation but were encountered more frequently in irradiated tumors than in non-irradiated ones. It is suggested that they may represent an unknown degenerative product of cytoplasm and/or nucleus rather than virus particles, despite their morphological resemblance to certain virus particles.
对可移植性小鼠神经母细胞瘤C 1300在X射线照射前后2小时至40天的不同时间间隔进行了超微结构研究。单次给予2000拉德和4000拉德X射线照射后，原本均匀的小肿瘤细胞在1至2天开始逐渐增大、多核化并退化。在5至7天，单核和多核巨细胞在小肿瘤细胞中占主导地位，而此后巨细胞逐渐消失，在10至14天小肿瘤细胞在巨细胞中占主导地位。巨细胞含有丰富的亚细胞细胞器，经X射线照射的肿瘤细胞显然在退化之前继续产生这些细胞器。在肿瘤细胞中观察到两种类型的细胞质颗粒，即池内A颗粒和棒状颗粒。池内A颗粒几乎存在于所有未照射的肿瘤细胞中，尽管其数量在细胞间差异很大，而在辐射诱导的巨细胞中观察到的频率较低，可能是由于稀释效应而非实际数量减少。棒状颗粒，神经母细胞瘤中迄今未描述过，直径为23纳米，长度可变，偶尔呈管状。它们仅出现在退化细胞中，无论是否接受X射线照射，但在照射后的肿瘤中比未照射的肿瘤中更常见。有人认为，尽管它们在形态上与某些病毒颗粒相似，但它们可能代表细胞质和/或细胞核的一种未知的退化产物，而非病毒颗粒。
