Hofmann G E, Scott R T, Bergh P A, Deligdisch L
Department of Obstetrics and Gynecology, Mount Sinai Medical Center, New York 10029.
J Clin Endocrinol Metab. 1991 Oct;73(4):882-7. doi: 10.1210/jcem-73-4-882.
Epidermal growth factor (EGF) was localized immunohistochemically in human endometrium throughout the menstrual cycle, in gestational decidua, and in first, second, and third trimester placenta using two polyclonal antihuman EGF antisera. In proliferative phase endometrium, moderate EGF immunostaining was localized to the cytoplasm of stromal cells, with absent to light staining of glandular epithelium. In the secretory phase, EGF immunostaining was intense and localized predominantly to stromal cells, particularly those surrounding spiral arterioles. There was absent to light EGF immunostaining within epithelial cells; however, there was no staining of subnuclear vacuoles. In addition, the luminal surface of exhausted secretory glands demonstrated moderate EGF immunostaining. In gestational decidua, EGF immunostaining was light to moderate in the stromal cells, but was intense in the surface epithelium. Intense EGF immunostaining was noted in the syncytiotrophoblast layer of first trimester placenta, with light to moderate staining of the cytotrophoblast. Immunostaining decreased in both layers of trophoblast as pregnancy progressed. Immunoreactive EGF is found in endometrium and trophoblast and may have a physiological role in endometrial and placental function.
使用两种多克隆抗人表皮生长因子（EGF）抗血清，通过免疫组织化学方法在整个月经周期的人子宫内膜、妊娠蜕膜以及妊娠早期、中期和晚期胎盘中定位EGF。在增殖期子宫内膜中，中度EGF免疫染色定位于基质细胞的细胞质，腺上皮染色缺失或淡染。在分泌期，EGF免疫染色强烈，主要定位于基质细胞，特别是围绕螺旋小动脉的那些细胞。上皮细胞内EGF免疫染色缺失或淡染；然而，核下空泡无染色。此外，耗竭分泌腺的腔表面显示中度EGF免疫染色。在妊娠蜕膜中，基质细胞中EGF免疫染色淡至中度，但表面上皮中染色强烈。在妊娠早期胎盘的合体滋养层中观察到强烈的EGF免疫染色，细胞滋养层染色淡至中度。随着妊娠进展，滋养层的两层染色均减弱。在子宫内膜和滋养层中发现了免疫反应性EGF，其可能在子宫内膜和胎盘功能中具有生理作用。
