Spitsin S V, Drobyshevskaya E I, Barkhatova O I, Belyi Y F, Tartakovsky I S, Nesterenko V G
Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Science, Moscow, USSR.
J Immunol. 1991 Sep 15;147(6):2001-5.
A panel of mAb (IgG1, IgG3, IgM) against Legionella pneumophila cytolysin (CL)-protease of 37 kDa was obtained. Subtyping of L. pneumophila strains of serogroup 1 by using mAb against CL (mAb-CL) was carried out. The results of comparative analysis of the specificity of mAb-CL and the panel of mAb kindly provided by Dr. J. M. Barbaree (Centers for Disease Control, Atlanta, GA) allowed us to recommend mAb-CL to be used as a diagnostic tool to reveal the pathogenicity of L. pneumophila strains of serogroup 1. Hybridomas were also raised in a syngenic system which produced anti-idiotypic mAb (mAb2) against anti-CL mAb B6/1. The Ab2 belonged to Ab2 gamma type: 1) Ab2 reacted with B6/1 Id only, 2) Ab2 inhibited the interaction of B6/1 Ab1 with CL, and 3) CL inhibited the reaction of Ab2 with Ab1. The use of Ab2 allowed us to show that B6/1 Id is expressed in 4 to 32% of serum antibodies during the primary and secondary immune responses of BALB/c mice to CL. Ab2 induced the production of anti-anti-idiotypic antibodies (Ab3) in BALB/c mice, and some of them reacted with CL. Thus, we have demonstrated the possibility of inducing an antibody response to CL (one of the main L. pneumophila pathogenic factors) in intact syngenic mice with anti-idiotypic antibodies.
获得了一组针对嗜肺军团菌细胞毒素（CL）-37 kDa蛋白酶的单克隆抗体（IgG1、IgG3、IgM）。使用针对CL的单克隆抗体（mAb-CL）对血清群1的嗜肺军团菌菌株进行了亚型分析。通过对mAb-CL与J. M. Barbaree博士（佐治亚州亚特兰大疾病控制中心）提供的单克隆抗体组的特异性进行比较分析，结果使我们推荐将mAb-CL用作揭示血清群1嗜肺军团菌菌株致病性的诊断工具。还在同基因系统中培养了杂交瘤，该系统产生了针对抗CL单克隆抗体B6/1的抗独特型单克隆抗体（mAb2）。该Ab2属于Ab2γ型：1）Ab2仅与B6/1独特型反应，2）Ab2抑制B6/1抗体1与CL的相互作用，3）CL抑制Ab2与抗体1的反应。使用Ab2使我们能够证明，在BALB/c小鼠对CL的初次和二次免疫反应期间，B6/1独特型在4%至32%的血清抗体中表达。Ab2在BALB/c小鼠中诱导产生抗抗独特型抗体（Ab3），其中一些与CL反应。因此，我们证明了用抗独特型抗体在完整的同基因小鼠中诱导针对CL（嗜肺军团菌主要致病因素之一）的抗体反应的可能性。
