Bardadin K A, Scheuer P J, Peczek A, Wejman J
Department of Histopathology, Royal Free Hospital School of Medicine, London, U.K.
J Pathol. 1991 Jul;164(3):253-9. doi: 10.1002/path.1711640311.
Phenotypic expression of macrophages was studied immunocytochemically in 25 human fetal livers at various stages of development and in 20 normal human adult livers. A panel of commercially available polyclonal and monoclonal antibodies (KP1, Mac387, LN3, CR3/43, and antibodies against muramidase, alpha-1-antitrypsin, and factor XIIIa) was applied to paraffin sections. From the seventh week of gestation macrophages in the fetal liver showed differences in distribution with the various antibodies. Macrophages in adult liver similarly varied in morphology and phenotypic expression. In the light of these results, we conclude that the population of human liver macrophages is heterogeneous from an early stage of fetal development and that this heterogeneity extends into adult life.
采用免疫细胞化学方法，对25例处于不同发育阶段的人胎儿肝脏以及20例正常成人肝脏中的巨噬细胞表型表达进行了研究。将一组市售的多克隆和单克隆抗体（KP1、Mac387、LN3、CR3/43，以及抗溶菌酶、α-1抗胰蛋白酶和因子XIIIa的抗体）应用于石蜡切片。从妊娠第7周起，胎儿肝脏中的巨噬细胞在不同抗体作用下呈现出分布差异。成人肝脏中的巨噬细胞在形态和表型表达上同样存在差异。根据这些结果，我们得出结论：人肝脏巨噬细胞群体从胎儿发育早期就具有异质性，且这种异质性持续至成年期。
