Bhoopat L, Taylor C R, Hofman F M
Clin Immunol Immunopathol. 1986 Nov;41(2):184-92. doi: 10.1016/0090-1229(86)90102-9.
Macrophage populations from human fetal liver were examined for the sequential appearance of different antigenic determinant during maturation. Frozen sections of liver, from 12 to 21 weeks gestation were analyzed using a series of four monoclonal antibodies with known specificity. The macrophage monoclonal antibodies used were OKM-1, which defines monocytes, macrophages, and granulocytes; Leu M-3 and MO-2, which identify monocytes and macrophages; and 6B8, a new macrophage monoclonal antibody which binds to tissue macrophages. The staining pattern described by each of these monoclonal reagents was compared with the distribution of morphologically distinguishable tissue macrophages in fetal liver, based on the expression of surface and/or cytoplasmic antigens. The data indicate that the antigens defined by OKM-1 and 6B8 are present on large numbers of cells as early as 12 weeks gestation. In contrast, the antigenic determinants identified by Leu M-3 and MO-2 are present only on cells in 15 to 21 weeks of gestation; thus these antigens are mature differentiation antigens. Furthermore, double-staining studies confirmed that with the increase in fetal age unique macrophage populations can be identified based on the matrix of antigenic determinants. Thus, macrophage heterogeneity in the fetal liver may be a function of maturation.
研究了来自人类胎儿肝脏的巨噬细胞群体在成熟过程中不同抗原决定簇的相继出现情况。使用一系列四种具有已知特异性的单克隆抗体，对妊娠12至21周的肝脏冰冻切片进行了分析。所使用的巨噬细胞单克隆抗体为OKM-1，它可识别单核细胞、巨噬细胞和粒细胞；Leu M-3和MO-2，可识别单核细胞和巨噬细胞；以及6B8，一种与组织巨噬细胞结合的新型巨噬细胞单克隆抗体。根据表面和/或细胞质抗原的表达情况，将这些单克隆试剂所描述的染色模式与胎儿肝脏中形态学上可区分的组织巨噬细胞的分布进行了比较。数据表明，早在妊娠12周时，由OKM-1和6B8所定义的抗原就存在于大量细胞上。相比之下，由Leu M-3和MO-2所识别的抗原决定簇仅存在于妊娠15至21周的细胞上；因此，这些抗原是成熟分化抗原。此外，双重染色研究证实，随着胎儿年龄的增加，基于抗原决定簇的组合可以识别出独特的巨噬细胞群体。因此，胎儿肝脏中的巨噬细胞异质性可能是成熟的一种表现。
