Wiedemann F, Link R, Pumpe K, Jacobshagen U, Schaefer H E, Wiesmüller K H, Hummel R P, Jung G, Bessler W, Böltz T
Institute of Immunobiology, University of Freiburg, F.R.G.
J Pathol. 1991 Jul;164(3):265-71. doi: 10.1002/path.1711640313.
The inflammatory reactions following subcutaneous application of adjuvants revealed characteristic pathological patterns. The injection of complete Freund's adjuvant (CFA) resulted in the formation of large lipid deposits encircled by an inflammatory reaction and concentrically arranged collagen bundles. Bacterial lipopolysaccharide (LPS) caused granulomatous aggregations of mononuclear cells with thrombotic vessel occlusions. Inoculation of the lipopeptide adjuvants induced accumulation of mononuclear cells with only minimal fibrotic changes which were resolved after day 28. Lipopeptide conjugates based on the head group tripalmitoyl-S-glyceryl-cysteinyl-serin (P3CS) can thus be used as effective immunogens and adjuvants without long-term tissue damage.
皮下注射佐剂后的炎症反应呈现出特征性的病理模式。注射完全弗氏佐剂（CFA）导致形成大的脂质沉积物，周围有炎症反应和同心排列的胶原束。细菌脂多糖（LPS）引起单核细胞肉芽肿性聚集并伴有血栓性血管闭塞。接种脂肽佐剂诱导单核细胞聚集，仅有轻微的纤维化改变，在第28天后消退。基于头基三棕榈酰-S-甘油基-半胱氨酰-丝氨酸（P3CS）的脂肽缀合物因此可以用作有效的免疫原和佐剂，而不会造成长期组织损伤。
