Korhonen M, Ylänne J, Laitinen L, Cooper H M, Quaranta V, Virtanen I
Department of Anatomy, University of Helsinki, Finland.
Lab Invest. 1991 Sep;65(3):347-56.
The distribution of the alpha 1-alpha 6 as well as alpha v, beta 1, beta 3 and beta 4 integrin subunits in human first and second trimester and term placentas was studied by indirect immunofluorescence microscopy using a panel of monoclonal antibodies (mAbs). In first and second trimester villi, the alpha 1 and beta 1 integrin subunits were detected in the stromal cells, that were mostly also immunoreactive for desmin. Desmin-positive stromal cells were also found in villi of term placentas, but the stroma was negative for anti-alpha 1 and -beta 1. In the villous trophoblast, anti-alpha 6 and -beta 4 revealed a distinct basal immunoreactivity during all stages of development, whereas immunoreactivity for the alpha 3 and beta 1 subunits emerged during the second and third trimesters. Throughout placental development, endothelia of villous capillaries reacted prominently with anti-alpha 1 and -beta 1. Intermediate trophoblastic cells displayed a somewhat heterogenous immunoreactivity for the beta 1, alpha 1, alpha 3 and alpha 5 integrin subunits, and differed from villous trophoblast also in their lack of expression of the alpha 6 and beta 4 subunits. While nondecidualized endometrial cells displayed weak reactivity for the alpha 1 and beta 1 integrin subunits, the individual decidual cells presented both a basement membrane and a cell surface-confined immunoreactivity for anti-alpha 1, -alpha 3, and -beta 1. The results suggest a role for integrins in placental development, and show that expression of integrins is modulated during the differentiation of trophoblast, villous stroma, and decidual cells. Furthermore, the basal localization of alpha 6 beta 4 and alpha 3 beta 1 integrins suggests that they are employed as basement membrane receptors in the villous trophoblast, and the emergence of the alpha 3 beta 1 complex may reflect that the cytotrophoblast and syncytiotrophoblast recognize the basement membrane differently.
利用一组单克隆抗体（mAb），通过间接免疫荧光显微镜技术研究了α1-α6以及αv、β1、β3和β4整合素亚单位在人早孕期、中孕期和足月胎盘的分布情况。在早孕期和中孕期绒毛中，α1和β1整合素亚单位在基质细胞中被检测到，这些基质细胞大多也对结蛋白呈免疫反应性。在足月胎盘绒毛中也发现了结蛋白阳性的基质细胞，但基质对抗α1和-β1呈阴性。在绒毛滋养层中，抗α6和-β4在发育的所有阶段均显示出明显的基底免疫反应性，而α3和β1亚单位的免疫反应性在孕中期和孕晚期出现。在整个胎盘发育过程中，绒毛毛细血管内皮细胞与抗α1和-β1反应强烈。中间滋养层细胞对β1、α1、α3和α5整合素亚单位表现出 somewhat heterogenous免疫反应性，并且在缺乏α6和β4亚单位表达方面也与绒毛滋养层不同。虽然未蜕膜化的子宫内膜细胞对α1和β1整合素亚单位反应较弱，但单个蜕膜细胞对抗α1、-α3和-β1既表现出基底膜又表现出细胞表面局限的免疫反应性。结果表明整合素在胎盘发育中起作用，并表明整合素的表达在滋养层、绒毛基质和蜕膜细胞的分化过程中受到调节。此外，α6β4和α3β1整合素的基底定位表明它们在绒毛滋养层中被用作基底膜受体以及α3β1复合物的出现可能反映细胞滋养层和合胞体滋养层对基底膜的识别不同。 （注：“somewhat heterogenous”这里可能有误，推测可能是“somewhat heterogeneous”，即“有些异质性的” ）
