Iwahashi M, Nakano R
Department of Obstetrics and Gynaecology, Wakayama Medical College, Japan.
J Clin Pathol. 1998 Jan;51(1):44-6. doi: 10.1136/jcp.51.1.44.
To provide some insight into the aetiology of spontaneous abortion, the contents of type V collagen was investigated in human decidual tissues in spontaneous abortion and normal pregnancy.
Collagens were extracted from decidual tissues in spontaneous abortion (n = 19) and normal pregnancy (n = 25). The different types of collagen alpha chains were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), stained with Coomassie brilliant blue, and measured by densitometry. The relative amounts of the alpha 1 (III) and alpha 1 (V) chains were calculated by dividing the band intensities of the alpha 1 (III) and alpha 1 (V) chains by that of the alpha 1 (I) chain.
The ratio of the alpha 1 (V) chain to that of the alpha 1 (I) chain in decidual tissues in spontaneous abortion was significantly lower than that found in normal pregnancy (p < 0.05).
These results suggest that type V collagen might play an important role in the maintenance of pregnancy and that decreased expression of this collagen could be associated with spontaneous abortion.
为深入了解自然流产的病因，对自然流产和正常妊娠的人蜕膜组织中V型胶原的含量进行了研究。
从自然流产（n = 19）和正常妊娠（n = 25）的蜕膜组织中提取胶原。不同类型的胶原α链通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分离，用考马斯亮蓝染色，并通过光密度测定法进行测量。α1（III）和α1（V）链的相对含量通过将α1（III）和α1（V）链的条带强度除以α1（I）链的条带强度来计算。
自然流产蜕膜组织中α1（V）链与α1（I）链的比值显著低于正常妊娠（p < 0.05）。
这些结果表明，V型胶原可能在维持妊娠中起重要作用，该胶原表达降低可能与自然流产有关。
