Tischler A S, McClain R M, Childers H, Downing J
Department of Pathology, Tufts University School of Medicine, Boston, Massachusetts.
Lab Invest. 1991 Sep;65(3):374-6.
The adrenal medulla is innervated by nerve fibers from several sources, which synapse on chromaffin cells and stimulate the secretion of catecholamines. The antihypertensive agent reserpine is known to reflexively increase this neurogenic stimulation by depleting catecholamine stores, and long-term administration of reserpine is associated with adrenal medullary hyperplasia and neoplasia. To determine the role of neurogenic signals in regulating normal and reserpine-stimulated proliferation of chromaffin cells, the incorporation of 5-bromo-2'-deoxyuridine (BrdU) into replicating nuclei was assessed in the adrenal medulla of adult rats. Unilateral adrenal denervation caused a 4-5 fold decrease in chromaffin cell labeling by 5-bromo-2'-deoxyuridine during a 2-week labeling period. Denervation also prevented stimulation of labeling in animals receiving reserpine in their diet. These findings suggest that neurogenic control of cell proliferation may play an important role in the pathogenesis of adrenal medullary hyperplasia and neoplasia, and in the normal development of the peripheral and central nervous systems.
肾上腺髓质由来自多个来源的神经纤维支配，这些神经纤维与嗜铬细胞形成突触并刺激儿茶酚胺的分泌。已知抗高血压药物利血平通过耗尽儿茶酚胺储备来反射性地增加这种神经源性刺激，长期服用利血平与肾上腺髓质增生和肿瘤形成有关。为了确定神经源性信号在调节嗜铬细胞正常和利血平刺激的增殖中的作用，在成年大鼠的肾上腺髓质中评估了5-溴-2'-脱氧尿苷（BrdU）掺入复制细胞核的情况。在为期2周的标记期内，单侧肾上腺去神经支配导致嗜铬细胞被5-溴-2'-脱氧尿苷标记的数量减少4至5倍。去神经支配还阻止了在饮食中接受利血平的动物中标记的刺激。这些发现表明，细胞增殖的神经源性控制可能在肾上腺髓质增生和肿瘤形成的发病机制以及外周和中枢神经系统的正常发育中起重要作用。
