Collins J M, Scott R B, McClish D K, Taylor J R, Grogan W M
Department of Biochemistry and Molecular Biophysics, Medical College, Virginia Commonwealth University, Richmond 23298.
Mech Ageing Dev. 1991 Jun 14;59(1-2):153-62. doi: 10.1016/0047-6374(91)90081-a.
Several reports have suggested that membrane rigidity, a term that refers to the relative motion of membrane constituents, is decreased in Alzheimer's Disease. Accordingly, a series of fluorescent membrane probes was used to evaluate the rigidity from the surface to the center of the outer hemi-leaflet of the plasma membrane of living neutrophils, monocytes and lymphocytes. Anisotropy, a parameter which increases with increasing membrane rigidity, was calculated from flow cytometric measurements of vertically and horizontally polarized components of the fluorescence emission of the probes. These preliminary experiments suggest that whereas membrane rigidity in certain regions of the plasma membrane of peripheral blood leukocytes is increased as expected in elderly controls, it is decreased in Alzheimer's disease.
几份报告表明，膜刚性（一个指膜成分相对运动的术语）在阿尔茨海默病中降低。因此，一系列荧光膜探针被用于评估活的中性粒细胞、单核细胞和淋巴细胞质膜外半层从表面到中心的刚性。各向异性是一个随膜刚性增加而增加的参数，它是根据探针荧光发射的垂直和水平偏振成分的流式细胞术测量计算得出的。这些初步实验表明，虽然外周血白细胞质膜某些区域的膜刚性如老年对照组预期的那样增加，但在阿尔茨海默病中却降低了。
