Piletz J E, Sarasua M, Whitehouse P, Chotani M
Department of Psychiatry, Case Western Reserve University, Cleveland, OH 44109.
Neurobiol Aging. 1991 Sep-Oct;12(5):401-6. doi: 10.1016/0197-4580(91)90064-q.
We studied 12 patients with probable Alzheimer's disease versus age- and sex-matched healthy control subjects. Platelets were subfractionated into intracellular membranes and plasma membranes, and steady-state anisotropy of diphenylhexatriene was measured on the preparations as an index of membrane fluidity. Fluidity was higher in intracellular membranes from platelets of Alzheimer's patients compared to controls (P = 0.016). However, no difference was observed in purified plasma membrane's fluidity from the same patients versus controls. Neither the platelet counts, platelet volumes, percent of larger platelets, nor the amount of internal membrane protein per platelet were different between groups. There was no correlation between intracellular membrane anisotropy and severity of dementia as measured on the Mini-Mental State Exam. The results extend previous studies suggesting that there is an intracellular membrane alteration in platelets in Alzheimer's disease.
我们研究了12例可能患有阿尔茨海默病的患者以及年龄和性别匹配的健康对照受试者。将血小板亚分级为细胞内膜和质膜，并测量制备物中二苯基己三烯的稳态各向异性，作为膜流动性的指标。与对照组相比，阿尔茨海默病患者血小板的细胞内膜流动性更高（P = 0.016）。然而，在同一患者的纯化质膜流动性与对照组之间未观察到差异。两组之间的血小板计数、血小板体积、较大血小板百分比以及每个血小板的内膜蛋白量均无差异。根据简易精神状态检查表测量，细胞内膜各向异性与痴呆严重程度之间没有相关性。这些结果扩展了先前的研究，表明阿尔茨海默病患者血小板存在细胞内膜改变。
