Brown D T, Riedel B
J Virol. 1977 Feb;21(2):601-9. doi: 10.1128/JVI.21.2.601-609.1977.
The morphogenesis of vesicular stomatitis virus was examined using freeze-fracture techniques, and the results obtained were compared with those from previously published experiments carried out with influenza viruses and togaviruses. The process of conversion of the host cell plasma membrane into the vesicular stomatitis virus envelope was accompanied by a loss of the intramembranal particles abundant in cell membranes. Frequently a dense accumulation of intramembranal particles could be seen at the base of the developing virion, suggesting that these structures might play some role in the generation of viral envelope. In addition to the viral structures that were seen to develop in the classical fashion, with their long axis perpendicular to the cell surface, structures were also found that suggested the initiation of a process similar to budding, with the long axis of the viral capsid parallel to the plasma membrane. In this situation, as in the "perpendicular" process, intramembranal particles were excluded from the viral structure, and an accumulation of these particles could be seen adjacent to the developing viral membrane.
利用冷冻断裂技术研究了水疱性口炎病毒的形态发生，并将所得结果与先前发表的关于流感病毒和披膜病毒的实验结果进行了比较。宿主细胞质膜转化为水疱性口炎病毒包膜的过程伴随着细胞膜中丰富的膜内颗粒的丢失。在发育中的病毒粒子基部经常可以看到膜内颗粒的密集堆积，这表明这些结构可能在病毒包膜的形成中起某种作用。除了以经典方式发育的病毒结构，其长轴垂直于细胞表面外，还发现了一些结构，提示启动了类似于出芽的过程，病毒衣壳的长轴与质膜平行。在这种情况下，与“垂直”过程一样，膜内颗粒被排除在病毒结构之外，并且在发育中的病毒膜附近可以看到这些颗粒的堆积。
