冷冻蚀刻中的膜分裂。共价结合的铁蛋白作为膜标记物。
Membrane splitting in freeze-ethching. Covalently bound ferritin as a membrane marker.
作者信息
Pinto da Silva P, Branton D
出版信息
J Cell Biol. 1970 Jun;45(3):598-605. doi: 10.1083/jcb.45.3.598.
Abstract
The freeze-etch technique was used to observe red blood cell ghosts labeled on both surfaces with covalently bound ferritin. Ferritin molecules were never observed on fracture faces, thus indicating that fracture does not show membrane-surface detail. Subliming away the surrounding ice did expose the ferritin on the membrane surface. These results were consistent with the concept that membranes split during the fracture process of freeze-etching.
摘要
采用冷冻蚀刻技术观察用共价结合铁蛋白标记两面的红细胞血影。在断裂面上从未观察到铁蛋白分子，因此表明断裂不能显示膜表面细节。升华去除周围的冰确实使膜表面的铁蛋白暴露出来。这些结果与冷冻蚀刻断裂过程中膜会裂开的概念一致。
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