Krasinski S D, Wheeler M B, Leiter A B
New England Medical Center Hospital, Tufts University School of Medicine, Boston, Massachusetts 02111.
Mol Endocrinol. 1991 Mar;5(3):433-40. doi: 10.1210/mend-5-3-433.
In the present study we describe the isolation, structural characterization, and developmental expression of the gene encoding the intestinal hormone peptide-YY. Examination of the nucleotide sequence of the peptide-YY gene reveals that each of the four exons encodes a functional domain of its mRNA that is analogous to the corresponding exons of the genes encoding two closely related peptides neuropeptide-Y and pancreatic polypeptide. The highly conserved structural organization of the genes encoding this family of three peptides suggests that each gene arose from the duplication of a common ancestral gene. Developmental studies reveal that the peptide-YY gene exhibits a complex pattern of tissue-specific expression in the gastrointestinal tract. Unlike many gastrointestinal hormones, peptide-YY mRNA levels are highest before birth. The pancreas appears to be the major site of peptide-YY gene expression in the fetus, exceeding colonic expression by 7-fold. The abundance of peptide-YY mRNA in the pancreas declines rapidly after birth, in contrast to the colon, where mRNA levels are maintained throughout development into adulthood. Expression of the peptide-YY gene before birth antedates the presence of known enteral secretagogues for this hormone, suggesting alternate mechanisms that control its biosynthesis during development.
在本研究中，我们描述了编码肠激素肽YY的基因的分离、结构特征及发育表达。对肽YY基因核苷酸序列的检测显示，四个外显子中的每一个都编码其mRNA的一个功能结构域，该结构域类似于编码两种密切相关肽神经肽Y和胰多肽的基因的相应外显子。编码这三种肽家族的基因高度保守的结构组织表明，每个基因都起源于一个共同祖先基因的复制。发育研究表明，肽YY基因在胃肠道呈现复杂的组织特异性表达模式。与许多胃肠激素不同，肽YY mRNA水平在出生前最高。胰腺似乎是胎儿中肽YY基因表达的主要部位，比结肠表达高出7倍。出生后胰腺中肽YY mRNA的丰度迅速下降，而结肠中的mRNA水平在整个发育过程直至成年期都保持稳定。出生前肽YY基因的表达早于该激素已知肠促分泌素的出现，这表明在发育过程中控制其生物合成的替代机制。
