Goumain M, Voisin T, Lorinet A M, Laburthe M
Unité de Neuroendocrinologie et Biologie Cellulaire Digestives, INSERM U410, Faculté de Médecine Xavier Bichat, Paris, France.
Biochem Biophys Res Commun. 1998 Jun 9;247(1):52-6. doi: 10.1006/bbrc.1998.8647.
Peptide YY (PYY), neuropeptide Y (NPY) and pancreatic polypeptide (PP) are structurally related peptides which have potent antisecretory effects in small and/or large intestines. Receptors mediating these effects are still unknown with the exception of a PYY-preferring receptor expressed in small intestinal crypts. In the present study, expression of recently cloned Y1, Y2, and Y5 receptors which have similar affinity for PYY and NPY and Y4 receptors which have a high affinity for PP was investigated in gut by RT-PCR analysis. The data show that all Y receptors are expressed in small intestine and/or colon but with specific distributions. Y1 receptors are only expressed in nonepithelial colonic tissue, whereas Y2 and Y4 receptors are present in both epithelial and nonepithelial tissue of the small or large intestine. In contrast, Y5 receptor expression appears to be restricted to epithelial crypts of the small intestine and nonepithelial tissue of colon. Sequencing of PCR products showed 100% identity with the corresponding sequences of the cloned Y1, Y4, or Y5 receptors. The PCR product obtained with Y2 primers from rat crypt cells showed 84% identity with the cloned human Y2 receptor. These data indicate a wide distribution of Y receptors in small intestine and colon. They also suggest that Y1, Y2, Y4, and Y5 receptors may be responsible for still unexplained effects of PYY, NPY, or PP on secretion in small and large intestines.
肽YY（PYY）、神经肽Y（NPY）和胰多肽（PP）是结构相关的肽类，它们在小肠和/或大肠中具有强大的抗分泌作用。除了在小肠隐窝中表达的一种优先结合PYY的受体外，介导这些作用的受体尚不清楚。在本研究中，通过逆转录聚合酶链反应（RT-PCR）分析，研究了最近克隆的对PYY和NPY具有相似亲和力的Y1、Y2和Y5受体以及对PP具有高亲和力的Y4受体在肠道中的表达情况。数据显示，所有Y受体均在小肠和/或结肠中表达，但具有特定的分布。Y1受体仅在上皮性结肠组织中表达，而Y2和Y4受体则存在于小肠或大肠的上皮和非上皮组织中。相比之下，Y5受体的表达似乎局限于小肠的上皮隐窝和结肠的非上皮组织。PCR产物的测序显示与克隆的Y1、Y4或Y5受体的相应序列具有100%的同一性。用大鼠隐窝细胞的Y2引物获得的PCR产物与克隆的人Y2受体具有84%的同一性。这些数据表明Y受体在小肠和结肠中广泛分布。它们还表明，Y1、Y2、Y4和Y5受体可能是PYY、NPY或PP对小肠和大肠分泌产生的仍未解释清楚的作用的原因。
