Larmie E T, Poston L
Department of Physiology, College of Medicine, University of Lagos, Nigeria.
Planta Med. 1991 Apr;57(2):116-8. doi: 10.1055/s-2006-960044.
The in vitro effects of griffonin and ouabain on erythrocyte sodium content have been investigated in 6 normal subjects and 6 sickle cell patients. Intracellular sodium contents of normal or sickle cells incubated for 8 h in tris buffer, griffonin/tris buffer, or ouabain/tris buffer were determined. Incubation of normal cells in tris buffer or 0.5 mmol/l griffonin had little effect on the cell sodium content. However, 1.0 mmol/l griffonin/tris buffer raised the cell sodium level (P less than 0.05) over the incubation period. Ouabain/tris buffer (0.5 mmol/l or 1.0 mmol/l) also raised the sodium content (P less than 0.05 to P less than 0.001). Incubation of sickle cells in tris buffer raised the cell sodium (P less than 0.05) as did 0.5 mmol/l or 1.0 mmol/l griffonin (P less than 0.05 to P less than 0.001). Ouabain/tris buffer (0.5 mmol/l or 1.0 mmol/l) raised the intra-erythrocyte sodium level (P less than 0.01 to P less than 0.001). These findings suggest that ouabain and griffonin both have similar actions on intra-erythrocyte sodium content although ouabain was more potent. It is suggested therefore that griffonin could be a useful anti-sickling drug for sickle cell disease crisis.
已在6名正常受试者和6名镰状细胞病患者中研究了格里芬宁和哇巴因对红细胞钠含量的体外作用。测定了在三羟甲基氨基甲烷缓冲液、格里芬宁/三羟甲基氨基甲烷缓冲液或哇巴因/三羟甲基氨基甲烷缓冲液中孵育8小时的正常或镰状细胞的细胞内钠含量。正常细胞在三羟甲基氨基甲烷缓冲液或0.5 mmol/L格里芬宁中孵育对细胞钠含量影响很小。然而，在孵育期间，1.0 mmol/L格里芬宁/三羟甲基氨基甲烷缓冲液使细胞钠水平升高（P<0.05）。哇巴因/三羟甲基氨基甲烷缓冲液（0.5 mmol/L或1.0 mmol/L）也使钠含量升高（P<0.05至P<0.001）。镰状细胞在三羟甲基氨基甲烷缓冲液中孵育使细胞钠升高（P<0.05），0.5 mmol/L或1.0 mmol/L格里芬宁孵育时也是如此（P<0.05至P<0.001）。哇巴因/三羟甲基氨基甲烷缓冲液（0.5 mmol/L或1.0 mmol/L）使红细胞内钠水平升高（P<0.01至P<0.001）。这些发现表明，哇巴因和格里芬宁对红细胞内钠含量具有相似的作用，尽管哇巴因的作用更强。因此，有人认为格里芬宁可能是治疗镰状细胞病危象的一种有用的抗镰变药物。
